Thermal inactivation of reduced ferredoxin (flavodoxin):NADP+oxidoreductase fromEscherichia coli
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چکیده
منابع مشابه
Reduction of cobamides by reduced ferredoxin.
Reduced ferredoxin is capable of reducing nonenzymatitally cyano-Bit. Spectral studies have shown that BE, (a l-electron reduced derivative of the vitamin) accumulates in the reaction although the formation of methyl-B12 was observed upon the addition of methyl iodide to the incubation. The data indicate that reduction of cyano-Blz by reduced ferredoxin results in the transient formation of B12...
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Clostridial ferredoxin and pyruvate-ferredoxin oxidoreductase activity was investigated after in vitro or in vivo treatment with sodium nitrite. In vitro treatment of commercially available Clostridium pasteurianum ferredoxin with sodium nitrite inhibited ferredoxin activity. Inhibition of ferredoxin activity increased with increasing levels of sodium nitrite. Ferredoxin was isolated from norma...
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Steady state rates of NADP reduction decline upon commencement of nitrite reduction in reconstituted chloroplast preparations. Similarly, steady state rates of nitrite reduction are lower, but not zero, during concurrent NADP reduction. These results imply that competition for substrate occurs and suggest that nitrite reduction can successfully compete for reduced ferredoxin, even at high rates...
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Cells of Escherichia coli ML308-225, harvested from the exponential phase, were heated in 50 mM potassium phosphate, and the loss in viability and inability to transport lactose, proline, and alpha-methylglucoside was compared. After cells were heated at 48 degrees C for 15 min, there was a 16% loss in viability and a similarly small reduction in the steady-state accumulation of lactose at 25 d...
متن کاملCluster and Fold Stability of E. coli ISC-Type Ferredoxin
Iron-sulfur clusters are essential protein prosthetic groups that provide their redox potential to several different metabolic pathways. Formation of iron-sulfur clusters is assisted by a specialised machine that comprises, among other proteins, a ferredoxin. As a first step to elucidate the precise role of this protein in cluster assembly, we have studied the factors governing the stability an...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2002
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(02)03349-5